Downstreaming Process

The stages carried out to isolate and purify an enzyme produced in a fermenter.

1. Growth of a large number of microorganisms
2. If enzymes are intracellular, they must be broken up first.
3. Enzymes are separated from incact cells, debris and other particles
4. Dilute impure solution os the enzyme (water is removed)
5. Concentrated, impure solution of the enzyme (impurities removed)
6. Concentrated purified solution or enzyme
7. Packaged
8. Distributed
9. Sold